Supplementary images

Copyright: Jugend forscht

Timm Piperīs
microcopy site
Introduction
Principles of VBDC
Principles of VPDC
Principles of VPBC
Materials and Methods
Results of VBDC
Results of VPDC
Results of VPBC
Further developments
in VBDC
Further developments
in VPDC
Further devepolments
in VPBC
Optical calculations
Discussion
Summarizing remarks
and conclusions
Supplementary
images
Video Downloads
References
List of Personal 
Publications
Patents
Acknowledgements
About me
Contact Details

In addition to the images shown in the Figures 1-64, several supplementary high resolution photomicrographs are presented 
showing some more examples of the use of my methods. All images shown in this homepage, are protected by copyright, 
but they may be downloaded for private use.



Scale of a Silver Angelfish (Pterophyllum scalare), horizontal field width (HFW): 0.16 mm,
brightfield (a), phase contrast (b), darkfield (c), condenser-based VBDC (d-f),
dominance of brightfield (d), equalized image (e), dominance of darkfield (f)


Diatom, diameter: 0.14 mm, objective 40x, brightfield (a), darkfield (b), phase contrast (c),
condenser-based concentric VBDC, relative dominance of brightfield (d), and darkfield (e)


Diatom, diameter: 0.12 mm, objective 40x, brightfield (a), standard darktfield (b),
eccentric condenser-based VBDC, contrast equalization with Photomatix Pro (c)


Diatoms, HFW: 0.16 mm, light stop-based axial darkfield, 40x mirror lens,
centric convex mirror used as a light stopper


Vitamin C, HFW: 0.20 mm, light stop-based axial darkfield, 40x mirror lens,
centric convex mirror used as a light stopper


Vitamin C, HFW: 0.16 mm, light stop-based axial darkfield, polarized light, lambda compensator,
40x mirror lens, centric convex mirror used as a light stopper






























Vitamin C, HFW: 0.20 mm, light stop-based axial darkfield, polarized light, quarter lambda compensator,
40x mirror lens, centric convex mirror used as a light stopper




Radiolarians, arranged slide (HFW: 1.0 mm), phase contrast (a), concentric-peripheral VPDC,
phase contrast-dominated (b), darkfield-dominated (c), standard darkfield (d)


Diatom (diameter: 0.12 mm), phase contrast (a), phase contrast-dominated VPDC ,
concentric illumination (b), darkfield-dominated VPDC, oblique illumination (c),
standard darkfield (d)




Alum crystallization (HFW: 1.4 mm), phase contrast (a), concentric-peripheral VPDC, 
phase contrast-dominated (b), darkfield-dominated (c), standard darkfield (d)




Marine bristle worm (Polychaeta ), HFW: 0.9 mm, normal darkfield (a), standard phase contrast (b),
axial VPDC, oblique illumination (c)





















Alum crystallization, HFW: 0.5 mm, phase contrast, (left) concentric-peripheral VPBC (right).




Vitamin C, HFW: 0.6 mm, brightfield (a), darkfield (b), phase contrast (c), axial VPBC (d)




Native unstained dental bacillus, HFW: 0.06 mm, standard darkfield (a), axial darkfield (b),
phase contrast (c), light stop-based VBDC (d),
computer-based simulation of axial VPDC
digital superposition of b and c (e).
Images a and c were taken with 100x oil immersion glass lenses, images b and d with a
125x catadioptric mirror objective (water immersion). In the latter cases, the backside
of the centric convex mirror was used as a light stopper for producing axial darkfield and
lightstop-based VBDC.
The digital sandwich (e) emphasizes  exraordinary high qualities which could achieved with
mirror lenses when fitted with an additional phae ring for axial VPDC.




Alum crystallization illuminated with incident light in darkfield (a) and brightfield (b), computer-simulation
of VBDC imaging based on incident light (c, superposition of a and b)
The images shown here were taken with a special incident light microscope used for examining semiconductors.
One epi-darkfield image (a) was superimposed with the corresponding epi-brightfield image (b)
using a computer to create a digital sandwich (c).
By superimposing both images a and b, the information of one brightfield image, i.e. the pyramidal shape in this example,
can be combined with information from the darkfield image, which in turn shows finer structures away from the
crystal in more detail. This example emphasizes the benefit which can be expected from redesigning epi-iluminators
for my methods-



Last Update: August 10th, 2012
Copyright: Timm Piper, 2012